Detailed information of TA system TA034253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4830638..4831240
• Replicon: chromosome
• Accession: NZ_AP022044
• Organism: Escherichia coli strain WP4-S17-ESBL-03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: H7R12_RS23505
• Protein ID: WP_000897302.1
• Coordinates: 4830929..4831240 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R12_RS23500
• Protein ID: WP_000356397.1
• Coordinates: 4830638..4830928 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R12_RS23470	4825945..4826874	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
H7R12_RS23475	4827056..4827298	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
H7R12_RS23480	4827588..4828436	+	849	WP_001038650.1	hypothetical protein	-
H7R12_RS23485	4828752..4829201	+	450	WP_032140890.1	hypothetical protein	-
H7R12_RS23490	4829386..4829604	-	219	WP_182913561.1	ribbon-helix-helix domain-containing protein	-
H7R12_RS23495	4830001..4830279	-	279	WP_001296612.1	hypothetical protein	-
H7R12_RS23500	4830638..4830928	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R12_RS23505	4830929..4831240	-	312	WP_000897302.1	hypothetical protein	Toxin
H7R12_RS23510	4831469..4832377	+	909	WP_001385591.1	alpha/beta hydrolase	-
H7R12_RS23515	4832441..4833382	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R12_RS23520	4833427..4833864	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R12_RS23525	4833861..4834733	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R12_RS23530	4834727..4835326	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T34253 WP_000897302.1 NZ_AP022044:c4831240-4830929 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T34253 NZ_AP022044:c4831240-4830929 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT34253 WP_000356397.1 NZ_AP022044:c4830928-4830638 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT34253 NZ_AP022044:c4830928-4830638 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416