Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1910449..1910951 Replicon chromosome
Accession NZ_AP022044
Organism Escherichia coli strain WP4-S17-ESBL-03

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag H7R12_RS09015 Protein ID WP_000767819.1
Coordinates 1910697..1910951 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag H7R12_RS09010 Protein ID WP_001259253.1
Coordinates 1910449..1910700 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R12_RS08985 1905627..1906694 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R12_RS08990 1906694..1907764 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
H7R12_RS08995 1907761..1909065 - 1305 WP_001296211.1 histidinol dehydrogenase -
H7R12_RS09000 1909071..1909970 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R12_RS09005 1910116..1910166 - 51 WP_001364200.1 his operon leader peptide -
H7R12_RS09010 1910449..1910700 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R12_RS09015 1910697..1910951 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R12_RS09020 1911034..1911858 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R12_RS09025 1911904..1912833 + 930 WP_000803366.1 LysR family transcriptional regulator -
H7R12_RS09030 1913048..1913110 + 63 WP_010723108.1 membrane protein YoeI -
H7R12_RS09035 1913100..1914458 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
H7R12_RS09040 1914675..1915133 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1914675..1915133 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T34234 WP_000767819.1 NZ_AP022044:1910697-1910951 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34234 NZ_AP022044:1910697-1910951 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT34234 WP_001259253.1 NZ_AP022044:1910449-1910700 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp

>AT34234 NZ_AP022044:1910449-1910700 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References