34214

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3615745..3616286 Replicon chromosome
Accession NZ_AP022036
Organism Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag H7R01_RS17370 Protein ID WP_012000916.1
Coordinates 3616008..3616286 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H7R01_RS17365 Protein ID WP_000729704.1
Coordinates 3615745..3616005 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R01_RS17340 3611528..3612313 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
H7R01_RS17345 3612285..3613997 + 1713 Protein_3391 flagellar biosynthesis protein FlhA -
H7R01_RS17350 3614102..3614380 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H7R01_RS17355 3614373..3614729 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
H7R01_RS17360 3614786..3615559 - 774 WP_000543899.1 C40 family peptidase -
H7R01_RS17365 3615745..3616005 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H7R01_RS17370 3616008..3616286 + 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H7R01_RS17375 3616442..3617182 + 741 WP_001225679.1 murein L,D-transpeptidase -
H7R01_RS17380 3617153..3617920 - 768 WP_000333380.1 class II glutamine amidotransferase -
H7R01_RS17385 3618126..3618704 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T34214 WP_012000916.1 NZ_AP022036:3616008-3616286 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T34214 NZ_AP022036:3616008-3616286 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT34214 WP_000729704.1 NZ_AP022036:3615745-3616005 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT34214 NZ_AP022036:3615745-3616005 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References