Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1859811..1860313 Replicon chromosome
Accession NZ_AP022036
Organism Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R01_RS08910 Protein ID WP_000767829.1
Coordinates 1860059..1860313 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R01_RS08905 Protein ID WP_001259255.1
Coordinates 1859811..1860062 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R01_RS08880 1854989..1856056 - 1068 WP_000080063.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R01_RS08885 1856056..1857126 - 1071 WP_000108915.1 histidinol-phosphate transaminase -
H7R01_RS08890 1857123..1858427 - 1305 WP_182912664.1 histidinol dehydrogenase -
H7R01_RS08895 1858433..1859332 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R01_RS08900 1859478..1859528 - 51 WP_001364200.1 his operon leader peptide -
H7R01_RS08905 1859811..1860062 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R01_RS08910 1860059..1860313 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R01_RS08915 1860396..1861220 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R01_RS08920 1861266..1862195 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R01_RS08925 1862410..1862472 + 63 WP_010723108.1 membrane protein YoeI -
H7R01_RS08930 1862462..1863820 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R01_RS08935 1863999..1865057 + 1059 WP_053289098.1 transport protein YeeE -
H7R01_RS08940 1865071..1865298 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34201 WP_000767829.1 NZ_AP022036:1860059-1860313 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34201 NZ_AP022036:1860059-1860313 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34201 WP_001259255.1 NZ_AP022036:1859811-1860062 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34201 NZ_AP022036:1859811-1860062 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References