34201

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1859811..1860313 Replicon chromosome
Accession NZ_AP022036
Organism Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R01_RS08910 Protein ID WP_000767829.1
Coordinates 1860059..1860313 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R01_RS08905 Protein ID WP_001259255.1
Coordinates 1859811..1860062 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R01_RS08880 1854989..1856056 - 1068 WP_000080063.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R01_RS08885 1856056..1857126 - 1071 WP_000108915.1 histidinol-phosphate transaminase -
H7R01_RS08890 1857123..1858427 - 1305 WP_182912664.1 histidinol dehydrogenase -
H7R01_RS08895 1858433..1859332 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R01_RS08900 1859478..1859528 - 51 WP_001364200.1 his operon leader peptide -
H7R01_RS08905 1859811..1860062 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R01_RS08910 1860059..1860313 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R01_RS08915 1860396..1861220 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R01_RS08920 1861266..1862195 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R01_RS08925 1862410..1862472 + 63 WP_010723108.1 membrane protein YoeI -
H7R01_RS08930 1862462..1863820 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R01_RS08935 1863999..1865057 + 1059 WP_053289098.1 transport protein YeeE -
H7R01_RS08940 1865071..1865298 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34201 WP_000767829.1 NZ_AP022036:1860059-1860313 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34201 NZ_AP022036:1860059-1860313 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34201 WP_001259255.1 NZ_AP022036:1859811-1860062 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34201 NZ_AP022036:1859811-1860062 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References