Detailed information of TA system TA034192

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79884..80123
• Replicon: plasmid pWP3-S18-ESBL-08_1
• Accession: NZ_AP022033
• Organism: Escherichia coli strain WP3-S18-ESBL-08

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: H7R15_RS24295
• Protein ID: WP_023144756.1
• Coordinates: 79989..80123 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79884..79944 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R15_RS24265	75675..76235	+	561	WP_000139341.1	fertility inhibition protein FinO	-
H7R15_RS24270	76366..76578	+	213	WP_013023861.1	hypothetical protein	-
H7R15_RS24275	77137..77562	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
H7R15_RS24280	77559..77909	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R15_RS24285	77940..79553	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
H7R15_RS24290	79631..79917	+	287	Protein_85	DUF2726 domain-containing protein	-
-	79884..79944	-	61	NuclAT_1	-	Antitoxin
-	79884..79944	-	61	NuclAT_1	-	Antitoxin
-	79884..79944	-	61	NuclAT_1	-	Antitoxin
-	79884..79944	-	61	NuclAT_1	-	Antitoxin
H7R15_RS24295	79989..80123	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R15_RS24300	80420..80674	+	255	WP_000083850.1	replication regulatory protein RepA	-
H7R15_RS24535	80780..80914	+	135	Protein_88	protein CopA/IncA	-
H7R15_RS24305	80911..80985	+	75	WP_031943482.1	RepA leader peptide Tap	-
H7R15_RS24310	80978..81835	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
H7R15_RS24315	82775..83428	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H7R15_RS24320	83521..83778	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H7R15_RS24325	83711..84112	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H7R15_RS24330	84361..84776	+	416	Protein_94	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-27	senB	1..105867	105867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T34192 WP_023144756.1 NZ_AP022033:79989-80123 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T34192 NZ_AP022033:79989-80123 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT34192 NZ_AP022033:c79944-79884 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin