Detailed information of TA system TA034157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3572333..3573027
• Replicon: chromosome
• Accession: NZ_AP022030
• Organism: Escherichia coli strain WP3-S18-ESBL-07

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H7R16_RS17280
• Protein ID: WP_001263489.1
• Coordinates: 3572333..3572731 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R16_RS17285
• Protein ID: WP_000554758.1
• Coordinates: 3572734..3573027 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3567921..3568001	-	81	NuclAT_12	-	-
-	3567921..3568001	-	81	NuclAT_12	-	-
-	3567921..3568001	-	81	NuclAT_12	-	-
-	3567921..3568001	-	81	NuclAT_12	-	-
H7R16_RS17255	3568597..3569055	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R16_RS17260	3569316..3570773	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H7R16_RS17265	3570830..3571351	-	522	Protein_3373	peptide chain release factor H	-
H7R16_RS17270	3571347..3571553	-	207	Protein_3374	RtcB family protein	-
H7R16_RS17275	3571871..3572323	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H7R16_RS17280	3572333..3572731	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R16_RS17285	3572734..3573027	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R16_RS17290	3573079..3574134	-	1056	WP_001226164.1	DNA polymerase IV	-
H7R16_RS17295	3574205..3574990	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H7R16_RS17300	3574962..3576674	+	1713	Protein_3380	flagellar biosynthesis protein FlhA	-
H7R16_RS17305	3576898..3577395	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T34157 WP_001263489.1 NZ_AP022030:c3572731-3572333 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T34157 NZ_AP022030:c3572731-3572333 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT34157 WP_000554758.1 NZ_AP022030:c3573027-3572734 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT34157 NZ_AP022030:c3573027-3572734 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3