Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1743759..1744261 Replicon chromosome
Accession NZ_AP022030
Organism Escherichia coli strain WP3-S18-ESBL-07

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag H7R16_RS08255 Protein ID WP_000767831.1
Coordinates 1744007..1744261 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R16_RS08250 Protein ID WP_001259255.1
Coordinates 1743759..1744010 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R16_RS08225 1738936..1740003 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R16_RS08230 1740003..1741073 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
H7R16_RS08235 1741070..1742374 - 1305 WP_001446927.1 histidinol dehydrogenase -
H7R16_RS08240 1742380..1743279 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R16_RS08245 1743425..1743475 - 51 WP_001364200.1 his operon leader peptide -
H7R16_RS08250 1743759..1744010 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R16_RS08255 1744007..1744261 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R16_RS08260 1744344..1745168 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R16_RS08265 1745214..1746143 + 930 WP_024195433.1 LysR family transcriptional regulator -
H7R16_RS08270 1746358..1746420 + 63 WP_010723108.1 membrane protein YoeI -
H7R16_RS08275 1746410..1747768 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R16_RS08280 1747947..1749005 + 1059 WP_000492321.1 transport protein YeeE -
H7R16_RS08285 1749019..1749246 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T34143 WP_000767831.1 NZ_AP022030:1744007-1744261 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T34143 NZ_AP022030:1744007-1744261 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34143 WP_001259255.1 NZ_AP022030:1743759-1744010 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34143 NZ_AP022030:1743759-1744010 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References