Detailed information of TA system TA034134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 159017..159744
• Replicon: plasmid pWP3-S18-ESBL-03_1
• Accession: NZ_AP022015
• Organism: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: H7R87_RS26720
• Protein ID: WP_000558568.1
• Coordinates: 159017..159328 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: H7R87_RS26725
• Protein ID: WP_000990392.1
• Coordinates: 159325..159744 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R87_RS26700	155531..156250	+	720	WP_001102107.1	(Fe-S)-binding protein	-
H7R87_RS26705	156261..157688	+	1428	WP_047693628.1	iron-sulfur cluster-binding protein	-
H7R87_RS26710	157681..158376	+	696	WP_000370412.1	lactate utilization protein C	-
H7R87_RS26715	158426..158806	-	381	Protein_158	gluconate permease	-
H7R87_RS26720	159017..159328	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R87_RS26725	159325..159744	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
H7R87_RS26730	159781..160983	-	1203	WP_047693625.1	tail stability protein	-
H7R87_RS26735	160976..161305	-	330	WP_000121260.1	hypothetical protein	-
H7R87_RS26740	161302..161961	-	660	WP_000078540.1	hypothetical protein	-
H7R87_RS26745	162364..162477	-	114	Protein_164	IS1 family transposase	-
H7R87_RS26750	162595..162759	-	165	Protein_165	EAL domain-containing protein	-
H7R87_RS26755	162901..163485	-	585	WP_047693620.1	LuxR family transcriptional regulator	-
H7R87_RS26760	163603..163851	-	249	Protein_167	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..346351	346351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T34134 WP_000558568.1 NZ_AP022015:159017-159328 [Klebsiella quasipneumoniae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T34134 NZ_AP022015:159017-159328 [Klebsiella quasipneumoniae]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT34134 WP_000990392.1 NZ_AP022015:159325-159744 [Klebsiella quasipneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT34134 NZ_AP022015:159325-159744 [Klebsiella quasipneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4