Detailed information of TA system TA034095

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72011..72264
• Replicon: plasmid pWP3-W18-ESBL-08_1
• Accession: NZ_AP022004
• Organism: Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: H7R22_RS25580
• Protein ID: WP_001336447.1
• Coordinates: 72115..72264 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72011..72067 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R22_RS25555	67913..68659	+	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-
H7R22_RS25560	68714..69271	+	558	WP_000139329.1	fertility inhibition protein FinO	-
H7R22_RS25565	70379..70840	+	462	WP_000760080.1	thermonuclease family protein	-
H7R22_RS25570	70943..71326	+	384	WP_001109264.1	hypothetical protein	-
H7R22_RS25575	71479..71916	+	438	WP_000872609.1	hypothetical protein	-
-	72011..72067	-	57	NuclAT_1	-	Antitoxin
-	72011..72067	-	57	NuclAT_1	-	Antitoxin
-	72011..72067	-	57	NuclAT_1	-	Antitoxin
-	72011..72067	-	57	NuclAT_1	-	Antitoxin
H7R22_RS25580	72115..72264	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R22_RS25585	72549..72806	+	258	WP_000084404.1	replication regulatory protein RepA	-
H7R22_RS25590	73040..73114	+	75	WP_001365705.1	RepA leader peptide Tap	-
H7R22_RS25595	73107..73964	+	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
H7R22_RS25600	74665..74877	+	213	WP_001336932.1	hypothetical protein	-
H7R22_RS25605	74992..75300	-	309	Protein_94	transposase	-
H7R22_RS25610	75493..75591	+	99	WP_137491101.1	transposase	-
H7R22_RS25615	75741..75989	-	249	WP_000738203.1	hypothetical protein	-
H7R22_RS25620	76019..76789	-	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..131334	131334
	-	flank	IS/Tn	-	-	74992..75258	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T34095 WP_001336447.1 NZ_AP022004:72115-72264 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T34095 NZ_AP022004:72115-72264 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT34095 NZ_AP022004:c72067-72011 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin