Detailed information of TA system TA034091

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29715..29985
• Replicon: plasmid pWP3-W18-ESBL-08_1
• Accession: NZ_AP022004
• Organism: Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R22_RS25335
• Protein ID: WP_001312861.1
• Coordinates: 29827..29985 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29715..29778 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R22_RS25305	25485..26012	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H7R22_RS25310	26070..26303	+	234	WP_000006003.1	DUF905 family protein	-
H7R22_RS25315	26364..28328	+	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
H7R22_RS25320	28397..28831	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H7R22_RS25325	28828..29547	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29559..29783	+	225	NuclAT_0	-	-
-	29559..29783	+	225	NuclAT_0	-	-
-	29559..29783	+	225	NuclAT_0	-	-
-	29559..29783	+	225	NuclAT_0	-	-
H7R22_RS25330	29568..29747	-	180	WP_001309233.1	hypothetical protein	-
-	29715..29778	-	64	-	-	Antitoxin
H7R22_RS25335	29827..29985	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R22_RS25340	30286..30490	-	205	Protein_41	pilus protein	-
H7R22_RS25345	30882..31178	+	297	WP_001545326.1	hypothetical protein	-
H7R22_RS25350	31288..32109	+	822	WP_001234445.1	DUF945 domain-containing protein	-
H7R22_RS25355	32406..33008	-	603	Protein_44	transglycosylase SLT domain-containing protein	-
H7R22_RS25360	33329..33712	+	384	WP_001151538.1	relaxosome protein TraM	-
H7R22_RS25365	33899..34588	+	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..131334	131334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T34091 WP_001312861.1 NZ_AP022004:29827-29985 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T34091 NZ_AP022004:29827-29985 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT34091 NZ_AP022004:c29778-29715 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin