Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4040997..4041691 Replicon chromosome
Accession NZ_AP022003
Organism Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag H7R22_RS19490 Protein ID WP_001263491.1
Coordinates 4040997..4041395 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag H7R22_RS19495 Protein ID WP_000554755.1
Coordinates 4041398..4041691 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R22_RS19460 4036166..4037410 - 1245 WP_000189541.1 esterase FrsA -
- 4036826..4036906 - 81 NuclAT_11 - -
- 4036826..4036906 - 81 NuclAT_11 - -
- 4036826..4036906 - 81 NuclAT_11 - -
- 4036826..4036906 - 81 NuclAT_11 - -
H7R22_RS19465 4037502..4037960 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H7R22_RS19470 4038221..4039678 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
H7R22_RS25910 4039735..4040087 - 353 Protein_3813 peptide chain release factor H -
H7R22_RS19480 4040083..4040289 - 207 Protein_3814 RtcB family protein -
H7R22_RS19485 4040535..4040987 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
H7R22_RS19490 4040997..4041395 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7R22_RS19495 4041398..4041691 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7R22_RS19500 4041743..4042798 - 1056 WP_001550655.1 DNA polymerase IV -
H7R22_RS19505 4042869..4043654 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
H7R22_RS19510 4043626..4045338 + 1713 Protein_3820 flagellar biosynthesis protein FlhA -
H7R22_RS19515 4045443..4045721 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H7R22_RS19520 4045714..4046070 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4030933..4041691 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T34082 WP_001263491.1 NZ_AP022003:c4041395-4040997 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T34082 NZ_AP022003:c4041395-4040997 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT34082 WP_000554755.1 NZ_AP022003:c4041691-4041398 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT34082 NZ_AP022003:c4041691-4041398 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References