Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1977227..1977729 Replicon chromosome
Accession NZ_AP022003
Organism Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R22_RS09385 Protein ID WP_000767829.1
Coordinates 1977475..1977729 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R22_RS09380 Protein ID WP_001259255.1
Coordinates 1977227..1977478 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R22_RS09355 1972405..1973472 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R22_RS09360 1973472..1974542 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7R22_RS09365 1974539..1975843 - 1305 WP_039267630.1 histidinol dehydrogenase -
H7R22_RS09370 1975849..1976748 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
H7R22_RS09375 1976894..1976944 - 51 WP_071593552.1 his operon leader peptide -
H7R22_RS09380 1977227..1977478 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R22_RS09385 1977475..1977729 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R22_RS09390 1977812..1978636 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R22_RS09395 1978682..1979611 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R22_RS09400 1979826..1979888 + 63 WP_010723108.1 membrane protein YoeI -
H7R22_RS09405 1979878..1981236 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R22_RS09410 1981415..1982473 + 1059 WP_023568741.1 transport protein YeeE -
H7R22_RS09415 1982487..1982714 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T34066 WP_000767829.1 NZ_AP022003:1977475-1977729 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T34066 NZ_AP022003:1977475-1977729 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT34066 WP_001259255.1 NZ_AP022003:1977227-1977478 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT34066 NZ_AP022003:1977227-1977478 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References