Detailed information of TA system TA034065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1212635..1213362
• Replicon: chromosome
• Accession: NZ_AP022003
• Organism: Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: H7R22_RS05845
• Protein ID: WP_000547555.1
• Coordinates: 1212635..1212946 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R22_RS05850
• Protein ID: WP_000126294.1
• Coordinates: 1212943..1213362 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R22_RS05820	1208570..1210279	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
H7R22_RS05825	1210289..1210831	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
H7R22_RS05830	1210831..1211598	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H7R22_RS05835	1211595..1212005	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
H7R22_RS05840	1211998..1212468	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
H7R22_RS05845	1212635..1212946	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R22_RS05850	1212943..1213362	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H7R22_RS05855	1213441..1214865	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
H7R22_RS05860	1214874..1216331	-	1458	WP_182914557.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H7R22_RS05865	1216591..1217601	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
H7R22_RS05870	1217750..1218277	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T34065 WP_000547555.1 NZ_AP022003:1212635-1212946 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T34065 NZ_AP022003:1212635-1212946 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT34065 WP_000126294.1 NZ_AP022003:1212943-1213362 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT34065 NZ_AP022003:1212943-1213362 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2