Detailed information of TA system TA034055

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79878..80117
• Replicon: plasmid pWP3-W18-ESBL-07_1
• Accession: NZ_AP021999
• Organism: Escherichia coli strain WP3-W18-ESBL-07

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: H7Q99_RS24640
• Protein ID: WP_023144756.1
• Coordinates: 79983..80117 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79878..79938 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7Q99_RS24610	75669..76229	+	561	WP_000139341.1	fertility inhibition protein FinO	-
H7Q99_RS24615	76360..76572	+	213	WP_013023861.1	hypothetical protein	-
H7Q99_RS24620	77131..77556	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
H7Q99_RS24625	77553..77903	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
H7Q99_RS24630	77934..79547	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
H7Q99_RS24635	79625..79911	+	287	Protein_85	DUF2726 domain-containing protein	-
-	79878..79938	-	61	NuclAT_1	-	Antitoxin
-	79878..79938	-	61	NuclAT_1	-	Antitoxin
-	79878..79938	-	61	NuclAT_1	-	Antitoxin
-	79878..79938	-	61	NuclAT_1	-	Antitoxin
H7Q99_RS24640	79983..80117	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7Q99_RS24645	80414..80668	+	255	WP_000083850.1	replication regulatory protein RepA	-
H7Q99_RS24930	80774..80908	+	135	Protein_88	protein CopA/IncA	-
H7Q99_RS24650	80905..80979	+	75	WP_031943482.1	RepA leader peptide Tap	-
H7Q99_RS24655	80972..81829	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
H7Q99_RS24660	82769..83422	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H7Q99_RS24665	83515..83772	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H7Q99_RS24670	83705..84106	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H7Q99_RS24675	84355..84770	+	416	Protein_94	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-27	senB	1..105864	105864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T34055 WP_023144756.1 NZ_AP021999:79983-80117 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T34055 NZ_AP021999:79983-80117 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT34055 NZ_AP021999:c79938-79878 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin