Detailed information of TA system TA033975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 689353..690089
• Replicon: chromosome
• Accession: NZ_AP021972
• Organism: Klebsiella sp. WP3-W18-ESBL-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R56_RS03405
• Protein ID: WP_106927099.1
• Coordinates: 689353..689670 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R56_RS03410
• Protein ID: WP_181358007.1
• Coordinates: 689673..690089 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R56_RS03385	685121..686089	-	969	WP_106927107.1	TerC family protein	-
H7R56_RS03390	686362..687348	-	987	WP_106927105.1	Gfo/Idh/MocA family oxidoreductase	-
H7R56_RS03395	687421..687921	-	501	WP_106927103.1	M48 family metallopeptidase	-
H7R56_RS03400	688005..689141	+	1137	WP_106927101.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
H7R56_RS03405	689353..689670	+	318	WP_106927099.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R56_RS03410	689673..690089	+	417	WP_181358007.1	helix-turn-helix domain-containing protein	Antitoxin
H7R56_RS03415	690093..692111	-	2019	WP_106927095.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
H7R56_RS03420	692257..693648	-	1392	WP_181358002.1	putrescine aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-136)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.16 Da
• Isoelectric Point: 9.9028

>T33975 WP_106927099.1 NZ_AP021972:689353-689670 [Klebsiella sp. WP3-W18-ESBL-02]
MHLISMAAIDEGCGRFPQFAKEIKLLGKVIAKANCPTPEALRAFYPSLDNFKYLDKHYVVNIANNNIRVIALIFFESQKF
YIRHIFNHAEYDRFTDKHRTKGNKS

Nucleotide

• Length: 318 bp

>T33975 NZ_AP021972:689353-689670 [Klebsiella sp. WP3-W18-ESBL-02]
ATGCATCTGATATCAATGGCTGCAATTGACGAGGGATGTGGCAGATTTCCCCAGTTTGCGAAAGAAATTAAGCTGTTAGG
TAAGGTGATTGCGAAAGCAAACTGTCCAACGCCAGAAGCGTTGAGGGCATTTTATCCCTCATTGGATAACTTCAAATATC
TTGATAAACACTATGTTGTGAACATTGCTAACAACAATATCAGGGTGATTGCGTTGATTTTTTTCGAAAGCCAGAAGTTT
TATATCCGGCACATTTTTAACCACGCTGAGTACGATAGGTTCACAGATAAACACAGAACGAAGGGGAATAAATCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15315.90 Da
• Isoelectric Point: 5.6982

>AT33975 WP_181358007.1 NZ_AP021972:689673-690089 [Klebsiella sp. WP3-W18-ESBL-02]
MMTAKALKATKTLVAEIPLLGDNPTEADHRDALELLAELLMDDPTNPLVDLLCVRIRAYEDNQPKLRALREEIEAIPTGI
AILRTLMEQHKLTLSDFADEIGSKSMVSRVLSGQRKLTLDHVKKLAARFGISPALFID

Nucleotide

• Length: 417 bp

>AT33975 NZ_AP021972:689673-690089 [Klebsiella sp. WP3-W18-ESBL-02]
ATGATGACGGCAAAAGCCCTGAAAGCCACAAAAACGCTGGTTGCTGAAATTCCTCTTCTGGGAGATAACCCCACAGAGGC
CGATCATCGTGACGCACTGGAACTGCTGGCTGAACTGCTGATGGACGACCCAACAAATCCGTTGGTTGATCTACTGTGCG
TTCGCATCCGTGCGTATGAGGACAACCAGCCGAAATTACGTGCGTTACGTGAAGAGATTGAGGCTATTCCCACGGGCATC
GCGATTTTGCGAACCCTGATGGAGCAGCATAAACTGACGCTTTCCGATTTTGCCGATGAGATTGGCAGCAAGTCGATGGT
TTCACGCGTACTCAGCGGTCAAAGAAAATTAACGCTGGATCATGTCAAAAAGCTGGCCGCTCGATTTGGCATTTCACCCG
CGTTATTTATTGATTAA