Detailed information of TA system TA033959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3799850..3800544
• Replicon: chromosome
• Accession: NZ_AP021963
• Organism: Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: H7R02_RS18375
• Protein ID: WP_001263500.1
• Coordinates: 3799850..3800248 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R02_RS18380
• Protein ID: WP_000554758.1
• Coordinates: 3800251..3800544 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R02_RS18350	3795215..3795673	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
H7R02_RS18355	3795934..3797391	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
H7R02_RS18360	3797448..3798062	-	615	WP_000602124.1	peptide chain release factor H	-
H7R02_RS18365	3798059..3799198	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
H7R02_RS18370	3799388..3799840	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
H7R02_RS18375	3799850..3800248	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R02_RS18380	3800251..3800544	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R02_RS18385	3800596..3801651	-	1056	WP_001226168.1	DNA polymerase IV	-
H7R02_RS18390	3801722..3802645	-	924	WP_032154006.1	putative lateral flagellar export/assembly protein LafU	-
H7R02_RS18395	3802648..3803511	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
H7R02_RS18400	3803524..3804240	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
H7R02_RS18405	3804260..3804727	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T33959 WP_001263500.1 NZ_AP021963:c3800248-3799850 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33959 NZ_AP021963:c3800248-3799850 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT33959 WP_000554758.1 NZ_AP021963:c3800544-3800251 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33959 NZ_AP021963:c3800544-3800251 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3