33956

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2530837..2531399 Replicon chromosome
Accession NZ_AP021963
Organism Escherichia coli strain WP3-W18-CRE-03

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H7R02_RS12115 Protein ID WP_000605675.1
Coordinates 2530837..2531115 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H7R02_RS12120 Protein ID WP_000781370.1
Coordinates 2531115..2531399 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R02_RS12090 2526423..2526854 - 432 WP_000152305.1 peroxiredoxin OsmC -
H7R02_RS12095 2527199..2527414 + 216 WP_000495766.1 biofilm-dependent modulation protein -
H7R02_RS12100 2527516..2527653 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H7R02_RS12105 2527810..2529507 + 1698 WP_000433460.1 malate dehydrogenase -
H7R02_RS12110 2529641..2530651 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
H7R02_RS12115 2530837..2531115 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R02_RS12120 2531115..2531399 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H7R02_RS12125 2531628..2532281 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
H7R02_RS12130 2532274..2533158 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H7R02_RS12135 2533171..2536218 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T33956 WP_000605675.1 NZ_AP021963:2530837-2531115 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T33956 NZ_AP021963:2530837-2531115 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT33956 WP_000781370.1 NZ_AP021963:2531115-2531399 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT33956 NZ_AP021963:2531115-2531399 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References