Detailed information of TA system TA033938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 59451..60178
• Replicon: plasmid pWP3-W18-CRE-02_2
• Accession: NZ_AP021952
• Organism: Klebsiella quasipneumoniae strain WP3-W18-CRE-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: H7R86_RS28040
• Protein ID: WP_000558568.1
• Coordinates: 59451..59762 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: H7R86_RS28045
• Protein ID: WP_000990392.1
• Coordinates: 59759..60178 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R86_RS28020	55965..56684	+	720	WP_001102107.1	(Fe-S)-binding protein	-
H7R86_RS28025	56695..58122	+	1428	WP_047693628.1	iron-sulfur cluster-binding protein	-
H7R86_RS28030	58115..58810	+	696	WP_000370412.1	lactate utilization protein C	-
H7R86_RS28035	58860..59240	-	381	Protein_57	gluconate permease	-
H7R86_RS28040	59451..59762	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R86_RS28045	59759..60178	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
H7R86_RS28050	60215..61417	-	1203	WP_047693625.1	tail stability protein	-
H7R86_RS28055	61410..61739	-	330	WP_000121260.1	hypothetical protein	-
H7R86_RS28060	61736..62395	-	660	WP_000078540.1	hypothetical protein	-
H7R86_RS28065	62798..62911	-	114	Protein_63	IS1 family transposase	-
H7R86_RS28070	63029..63193	-	165	Protein_64	EAL domain-containing protein	-
H7R86_RS28075	63335..63919	-	585	WP_047693620.1	LuxR family transcriptional regulator	-
H7R86_RS28080	64037..64285	-	249	Protein_66	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Il / blaGES-4 / catB8 / aac(6')-31 / qacE / sul1	-	1..206811	206811

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T33938 WP_000558568.1 NZ_AP021952:59451-59762 [Klebsiella quasipneumoniae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T33938 NZ_AP021952:59451-59762 [Klebsiella quasipneumoniae]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT33938 WP_000990392.1 NZ_AP021952:59759-60178 [Klebsiella quasipneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT33938 NZ_AP021952:59759-60178 [Klebsiella quasipneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4