Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 90220..90484 Replicon plasmid pWP2-S18-ESBL-08_2
Accession NZ_AP021948
Organism Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag H7R07_RS26575 Protein ID WP_001303307.1
Coordinates 90332..90484 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 90220..90282 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R07_RS26560 85459..87750 - 2292 WP_024255997.1 hypothetical protein -
H7R07_RS26565 87743..88813 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
H7R07_RS26570 88832..90040 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 90220..90282 - 63 NuclAT_0 - Antitoxin
- 90220..90282 - 63 NuclAT_0 - Antitoxin
- 90220..90282 - 63 NuclAT_0 - Antitoxin
- 90220..90282 - 63 NuclAT_0 - Antitoxin
H7R07_RS26575 90332..90484 + 153 WP_001303307.1 Hok/Gef family protein Toxin
H7R07_RS26580 90556..90807 - 252 WP_001291965.1 hypothetical protein -
- 91194..91245 - 52 NuclAT_1 - -
- 91194..91245 - 52 NuclAT_1 - -
- 91194..91245 - 52 NuclAT_1 - -
- 91194..91245 - 52 NuclAT_1 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-8 - 1..91630 91630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T33919 WP_001303307.1 NZ_AP021948:90332-90484 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T33919 NZ_AP021948:90332-90484 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT33919 NZ_AP021948:c90282-90220 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References