Detailed information of TA system TA033913

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134396..134822
• Replicon: plasmid pWP2-S18-ESBL-08_1
• Accession: NZ_AP021947
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H7R07_RS25665
• Protein ID: WP_001302184.1
• Coordinates: 134396..134554 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	134598..134822 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R07_RS25635	129558..129953	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
H7R07_RS25640	130063..130752	-	690	WP_000283384.1	conjugal transfer transcriptional regulator TraJ	-
H7R07_RS25645	130939..131322	-	384	WP_001151524.1	relaxosome protein TraM	-
H7R07_RS25650	131643..132245	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H7R07_RS25655	132542..133363	-	822	WP_001234487.1	DUF945 domain-containing protein	-
H7R07_RS25660	133474..133770	-	297	WP_001272245.1	hypothetical protein	-
H7R07_RS25665	134396..134554	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	134598..134822	-	225	NuclAT_0	-	Antitoxin
-	134598..134822	-	225	NuclAT_0	-	Antitoxin
-	134598..134822	-	225	NuclAT_0	-	Antitoxin
-	134598..134822	-	225	NuclAT_0	-	Antitoxin
H7R07_RS25670	134634..134813	+	180	WP_001334662.1	hypothetical protein	-
H7R07_RS25675	134834..135553	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
H7R07_RS25680	135550..135984	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
H7R07_RS25685	136053..138017	-	1965	WP_068862930.1	ParB/RepB/Spo0J family partition protein	-
H7R07_RS25690	138081..138314	-	234	WP_000005988.1	DUF905 family protein	-
H7R07_RS25695	138277..138909	-	633	WP_039023645.1	single-stranded DNA-binding protein	-
H7R07_RS25700	139210..139635	+	426	WP_015387398.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..210712	210712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T33913 WP_001302184.1 NZ_AP021947:c134554-134396 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T33913 NZ_AP021947:c134554-134396 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT33913 NZ_AP021947:c134822-134598 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin