Detailed information of TA system TA033908

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96265..96519
• Replicon: plasmid pWP2-S18-ESBL-08_1
• Accession: NZ_AP021947
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H7R07_RS25445
• Protein ID: WP_001351576.1
• Coordinates: 96265..96471 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96458..96519 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R07_RS25405	91804..92151	+	348	Protein_90	IS1-like element IS1A family transposase	-
H7R07_RS25410	92591..92878	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R07_RS25415	92875..93126	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H7R07_RS25420	93199..93390	-	192	WP_053270994.1	hypothetical protein	-
H7R07_RS25425	94093..94950	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H7R07_RS25430	94943..95425	-	483	WP_001273588.1	hypothetical protein	-
H7R07_RS25435	95418..95492	-	75	WP_001442103.1	RepA leader peptide Tap	-
H7R07_RS25440	95724..95981	-	258	WP_000083845.1	replication regulatory protein RepA	-
H7R07_RS25445	96265..96471	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96458..96519	+	62	NuclAT_1	-	Antitoxin
-	96458..96519	+	62	NuclAT_1	-	Antitoxin
-	96458..96519	+	62	NuclAT_1	-	Antitoxin
-	96458..96519	+	62	NuclAT_1	-	Antitoxin
H7R07_RS25450	96775..96849	-	75	Protein_99	endonuclease	-
H7R07_RS25455	97095..97307	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H7R07_RS25460	97443..98003	-	561	WP_032072881.1	fertility inhibition protein FinO	-
H7R07_RS25465	98106..98966	-	861	WP_000704513.1	alpha/beta hydrolase	-
H7R07_RS25470	99025..99771	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..210712	210712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T33908 WP_001351576.1 NZ_AP021947:c96471-96265 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T33908 NZ_AP021947:c96471-96265 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT33908 NZ_AP021947:96458-96519 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin