Detailed information of TA system TA033897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3878173..3878867
• Replicon: chromosome
• Accession: NZ_AP021946
• Organism: Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H7R07_RS18980
• Protein ID: WP_001263489.1
• Coordinates: 3878173..3878571 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R07_RS18985
• Protein ID: WP_000554758.1
• Coordinates: 3878574..3878867 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3873761..3873841	-	81	NuclAT_12	-	-
-	3873761..3873841	-	81	NuclAT_12	-	-
-	3873761..3873841	-	81	NuclAT_12	-	-
-	3873761..3873841	-	81	NuclAT_12	-	-
H7R07_RS18955	3874437..3874895	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R07_RS18960	3875156..3876613	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H7R07_RS18965	3876670..3877191	-	522	Protein_3716	peptide chain release factor H	-
H7R07_RS18970	3877187..3877393	-	207	Protein_3717	RtcB family protein	-
H7R07_RS18975	3877711..3878163	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H7R07_RS18980	3878173..3878571	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R07_RS18985	3878574..3878867	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R07_RS18990	3878919..3879974	-	1056	WP_001226164.1	DNA polymerase IV	-
H7R07_RS18995	3880045..3880830	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H7R07_RS19000	3880802..3882514	+	1713	Protein_3723	flagellar biosynthesis protein FlhA	-
H7R07_RS19005	3882738..3883235	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3818225..3879974	61749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T33897 WP_001263489.1 NZ_AP021946:c3878571-3878173 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33897 NZ_AP021946:c3878571-3878173 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT33897 WP_000554758.1 NZ_AP021946:c3878867-3878574 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33897 NZ_AP021946:c3878867-3878574 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3