Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1998018..1998520 Replicon chromosome
Accession NZ_AP021946
Organism Escherichia coli strain WP2-S18-ESBL-08

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R07_RS09570 Protein ID WP_000767829.1
Coordinates 1998266..1998520 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R07_RS09565 Protein ID WP_001259255.1
Coordinates 1998018..1998269 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R07_RS09540 1993195..1994262 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R07_RS09545 1994262..1995332 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
H7R07_RS09550 1995329..1996633 - 1305 WP_001446927.1 histidinol dehydrogenase -
H7R07_RS09555 1996639..1997538 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R07_RS09560 1997684..1997734 - 51 WP_001364200.1 his operon leader peptide -
H7R07_RS09565 1998018..1998269 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R07_RS09570 1998266..1998520 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R07_RS09575 1998603..1999427 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R07_RS09580 1999473..2000402 + 930 WP_000803366.1 LysR family transcriptional regulator -
H7R07_RS09585 2000617..2000679 + 63 WP_010723108.1 membrane protein YoeI -
H7R07_RS09590 2000669..2002027 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R07_RS09595 2002206..2003264 + 1059 WP_000492331.1 transport protein YeeE -
H7R07_RS09600 2003278..2003505 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33884 WP_000767829.1 NZ_AP021946:1998266-1998520 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33884 NZ_AP021946:1998266-1998520 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33884 WP_001259255.1 NZ_AP021946:1998018-1998269 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33884 NZ_AP021946:1998018-1998269 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References