Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1918349..1918851 Replicon chromosome
Accession NZ_AP021944
Organism Escherichia coli strain WP2-S18-ESBL-07

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R05_RS09220 Protein ID WP_000767829.1
Coordinates 1918597..1918851 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R05_RS09215 Protein ID WP_001259255.1
Coordinates 1918349..1918600 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R05_RS09190 1913527..1914594 - 1068 WP_096970764.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R05_RS09195 1914594..1915664 - 1071 WP_089590514.1 histidinol-phosphate transaminase -
H7R05_RS09200 1915661..1916965 - 1305 WP_001358504.1 histidinol dehydrogenase -
H7R05_RS09205 1916971..1917870 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
H7R05_RS09210 1918016..1918066 - 51 WP_001364200.1 his operon leader peptide -
H7R05_RS09215 1918349..1918600 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R05_RS09220 1918597..1918851 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R05_RS09225 1918934..1919758 + 825 WP_000754752.1 SDR family oxidoreductase -
H7R05_RS09230 1919804..1920733 + 930 WP_000803366.1 LysR family transcriptional regulator -
H7R05_RS09235 1920948..1921010 + 63 WP_010723108.1 membrane protein YoeI -
H7R05_RS09240 1921000..1922358 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R05_RS09245 1922537..1923595 + 1059 WP_104692549.1 transport protein YeeE -
H7R05_RS09250 1923609..1923836 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33854 WP_000767829.1 NZ_AP021944:1918597-1918851 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33854 NZ_AP021944:1918597-1918851 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33854 WP_001259255.1 NZ_AP021944:1918349-1918600 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33854 NZ_AP021944:1918349-1918600 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References