Detailed information of TA system TA033846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 37041..37693
• Replicon: plasmid pWP2-S18-CRE-03_1
• Accession: NZ_AP021941
• Organism: Aeromonas veronii strain WP2-S18-CRE-03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6I4WW70
• Locus tag: H7R57_RS23085
• Protein ID: WP_024943279.1
• Coordinates: 37041..37391 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R57_RS23090
• Protein ID: WP_024943280.1
• Coordinates: 37391..37693 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R57_RS23050	33163..33402	+	240	WP_042044878.1	CopG family ribbon-helix-helix protein	-
H7R57_RS23055	33392..33676	+	285	WP_139731036.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R57_RS23060	33948..34049	+	102	Protein_39	IS5/IS1182 family transposase	-
H7R57_RS23065	34577..35278	+	702	WP_182928087.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
H7R57_RS23070	35445..35657	+	213	WP_182928038.1	hypothetical protein	-
H7R57_RS23075	35679..36596	+	918	WP_182928039.1	IS5 family transposase	-
H7R57_RS23080	36737..36982	+	246	WP_160839242.1	hypothetical protein	-
H7R57_RS23085	37041..37391	+	351	WP_024943279.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R57_RS23090	37391..37693	+	303	WP_024943280.1	helix-turn-helix transcriptional regulator	Antitoxin
H7R57_RS23095	38120..38497	+	378	WP_182928040.1	hypothetical protein	-
H7R57_RS23100	38500..39318	+	819	WP_182928041.1	transposase	-
H7R57_RS23105	39352..42450	-	3099	WP_182928042.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-5.1	-	1..69526	69526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12937.79 Da
• Isoelectric Point: 5.7350

>T33846 WP_024943279.1 NZ_AP021941:37041-37391 [Aeromonas veronii]
MWTVKTTSLFDVWFDEQDEATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGDPLRAFFAFDPLRQAI
VLCAGNKGGNDKRFYKEMLPVAEAEYAKHLTELEVG

Nucleotide

• Length: 351 bp

>T33846 NZ_AP021941:37041-37391 [Aeromonas veronii]
ATGTGGACAGTAAAAACGACCTCTCTATTCGATGTCTGGTTTGATGAACAAGACGAGGCCACGCAGGAGAAGGTTTTGGC
TGGGTTGCTTGCCTTGGCACAGGGCGGTCCATCAGTAGGGCGACCCTTAGTTGATACCATCAAGGGGTCTCGCTTTACGA
ACCTCAAAGAGCTGAGAGTTCAGCACCAGGGGGATCCGCTACGAGCGTTCTTTGCTTTTGACCCGTTGCGGCAAGCTATC
GTGTTGTGTGCTGGAAATAAGGGCGGTAACGATAAGCGGTTTTACAAAGAGATGTTGCCGGTCGCTGAGGCGGAATACGC
CAAGCACTTAACTGAGTTGGAGGTAGGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11137.84 Da
• Isoelectric Point: 5.1667

>AT33846 WP_024943280.1 NZ_AP021941:37391-37693 [Aeromonas veronii]
MGRTLNEMLASRSVDSQQRIQEMANELLLEVRLQALREELEVSQVELASALGISQPAVVAMEQRGNDIKLSTMKRYVEAM
GGKLRVDVELPTGRHVGFNV

Nucleotide

• Length: 303 bp

>AT33846 NZ_AP021941:37391-37693 [Aeromonas veronii]
ATGGGAAGAACACTGAATGAGATGCTGGCCTCCCGCTCGGTGGATAGTCAGCAGCGGATCCAGGAGATGGCTAACGAGCT
GCTGCTGGAGGTACGCTTGCAAGCGCTGCGTGAAGAGTTGGAAGTGTCCCAGGTGGAGTTGGCCTCTGCCCTCGGGATCA
GCCAACCGGCCGTTGTGGCGATGGAGCAGCGTGGGAATGATATCAAGCTATCCACCATGAAGCGTTACGTGGAAGCAATG
GGTGGGAAGCTGCGTGTTGATGTTGAGCTTCCTACAGGGAGGCATGTCGGTTTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A6I4WW70