Detailed information of TA system TA033842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4788956..4789558
• Replicon: chromosome
• Accession: NZ_AP021935
• Organism: Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7X62_RS22540
• Protein ID: WP_000897305.1
• Coordinates: 4789247..4789558 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7X62_RS22535
• Protein ID: WP_000356395.1
• Coordinates: 4788956..4789246 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X62_RS22500	4784580..4785482	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7X62_RS22505	4785479..4786114	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7X62_RS22510	4786111..4787040	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H7X62_RS22515	4787222..4787464	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
H7X62_RS22520	4787683..4787901	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7X62_RS22525	4788320..4788598	-	279	WP_001315112.1	hypothetical protein	-
H7X62_RS22530	4788650..4788871	-	222	WP_001550354.1	hypothetical protein	-
H7X62_RS22535	4788956..4789246	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
H7X62_RS22540	4789247..4789558	-	312	WP_000897305.1	hypothetical protein	Toxin
H7X62_RS22545	4789787..4790695	+	909	WP_001550353.1	alpha/beta hydrolase	-
H7X62_RS22550	4790863..4792677	-	1815	WP_001550352.1	hypothetical protein	-
H7X62_RS22555	4793093..4794034	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7X62_RS22560	4794079..4794516	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T33842 WP_000897305.1 NZ_AP021935:c4789558-4789247 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T33842 NZ_AP021935:c4789558-4789247 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT33842 WP_000356395.1 NZ_AP021935:c4789246-4788956 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT33842 NZ_AP021935:c4789246-4788956 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7