Detailed information of TA system TA033835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3744692..3745419
• Replicon: chromosome
• Accession: NZ_AP021935
• Organism: Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: H7X62_RS17580
• Protein ID: WP_000547555.1
• Coordinates: 3745108..3745419 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7X62_RS17575
• Protein ID: WP_000126294.1
• Coordinates: 3744692..3745111 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7X62_RS17555	3739777..3740304	-	528	WP_001078777.1	electron transport protein HydN	-
H7X62_RS17560	3740453..3741463	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
H7X62_RS17565	3741723..3743180	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H7X62_RS17570	3743189..3744613	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
H7X62_RS17575	3744692..3745111	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H7X62_RS17580	3745108..3745419	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7X62_RS17585	3745586..3746056	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
H7X62_RS17590	3746049..3746459	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
H7X62_RS17595	3746456..3747223	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H7X62_RS17600	3747223..3747765	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
H7X62_RS17605	3747775..3749484	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T33835 WP_000547555.1 NZ_AP021935:c3745419-3745108 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T33835 NZ_AP021935:c3745419-3745108 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT33835 WP_000126294.1 NZ_AP021935:c3745111-3744692 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT33835 NZ_AP021935:c3745111-3744692 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2