Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1035833..1036527 Replicon chromosome
Accession NZ_AP021935
Organism Escherichia coli strain WP2-W18-ESBL-10

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag H7X62_RS04905 Protein ID WP_001263491.1
Coordinates 1036129..1036527 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag H7X62_RS04900 Protein ID WP_000554755.1
Coordinates 1035833..1036126 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7X62_RS04875 1031454..1031810 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H7X62_RS04880 1031803..1032081 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H7X62_RS04885 1032186..1033898 - 1713 Protein_951 flagellar biosynthesis protein FlhA -
H7X62_RS04890 1033870..1034655 + 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
H7X62_RS04895 1034726..1035781 + 1056 WP_001550655.1 DNA polymerase IV -
H7X62_RS04900 1035833..1036126 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7X62_RS04905 1036129..1036527 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7X62_RS04910 1036537..1036989 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
H7X62_RS04915 1037235..1037441 + 207 Protein_957 RtcB family protein -
H7X62_RS23495 1037437..1037789 + 353 Protein_958 peptide chain release factor H -
H7X62_RS04925 1037846..1039303 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
H7X62_RS04930 1039564..1040022 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H7X62_RS04935 1040114..1041358 + 1245 WP_000189541.1 esterase FrsA -
- 1040618..1040698 + 81 NuclAT_8 - -
- 1040618..1040698 + 81 NuclAT_8 - -
- 1040618..1040698 + 81 NuclAT_8 - -
- 1040618..1040698 + 81 NuclAT_8 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T33818 WP_001263491.1 NZ_AP021935:1036129-1036527 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T33818 NZ_AP021935:1036129-1036527 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT33818 WP_000554755.1 NZ_AP021935:1035833-1036126 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT33818 NZ_AP021935:1035833-1036126 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References