Detailed information of TA system TA033811

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69759..70013
• Replicon: plasmid pWP2-W18-ESBL-08_1
• Accession: NZ_AP021934
• Organism: Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H7R10_RS23700
• Protein ID: WP_001351576.1
• Coordinates: 69807..70013 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69759..69820 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R10_RS23675	66507..67253	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
H7R10_RS23680	67312..68172	+	861	WP_000704514.1	alpha/beta hydrolase	-
H7R10_RS23685	68275..68835	+	561	WP_000139315.1	fertility inhibition protein FinO	-
H7R10_RS23690	68971..69183	+	213	WP_001299730.1	ANR family transcriptional regulator	-
H7R10_RS23695	69429..69503	+	75	Protein_76	endonuclease	-
-	69759..69820	-	62	NuclAT_1	-	Antitoxin
-	69759..69820	-	62	NuclAT_1	-	Antitoxin
-	69759..69820	-	62	NuclAT_1	-	Antitoxin
-	69759..69820	-	62	NuclAT_1	-	Antitoxin
H7R10_RS23700	69807..70013	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R10_RS23705	70297..70554	+	258	WP_000083845.1	replication regulatory protein RepA	-
H7R10_RS23710	70786..70860	+	75	WP_001442103.1	RepA leader peptide Tap	-
H7R10_RS23715	70853..71335	+	483	WP_001273588.1	hypothetical protein	-
H7R10_RS23720	71328..72185	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H7R10_RS23725	73124..73777	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H7R10_RS23730	73870..74127	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H7R10_RS23735	74060..74461	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / blaCTX-M-65 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	iucA / iucB / iucC / iucD / iutA	1..105973	105973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T33811 WP_001351576.1 NZ_AP021934:69807-70013 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T33811 NZ_AP021934:69807-70013 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT33811 NZ_AP021934:c69820-69759 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin