Detailed information of TA system TA033806

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31414..31684
• Replicon: plasmid pWP2-W18-ESBL-08_1
• Accession: NZ_AP021934
• Organism: Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R10_RS23480
• Protein ID: WP_001312861.1
• Coordinates: 31526..31684 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31414..31477 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R10_RS23440	26634..27605	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
H7R10_RS23445	28744..28950	+	207	WP_000275856.1	hypothetical protein	-
H7R10_RS23450	28976..29515	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
H7R10_RS23455	29583..29816	+	234	WP_000005987.1	DUF905 family protein	-
H7R10_RS23460	29844..30041	+	198	Protein_29	hypothetical protein	-
H7R10_RS23465	30096..30530	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
H7R10_RS23470	30527..31246	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
H7R10_RS23475	31258..31446	-	189	WP_001299721.1	hypothetical protein	-
-	31258..31482	+	225	NuclAT_0	-	-
-	31258..31482	+	225	NuclAT_0	-	-
-	31258..31482	+	225	NuclAT_0	-	-
-	31258..31482	+	225	NuclAT_0	-	-
-	31414..31477	-	64	-	-	Antitoxin
H7R10_RS23480	31526..31684	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R10_RS23485	32605..32892	+	288	WP_000107535.1	hypothetical protein	-
H7R10_RS23490	33010..33831	+	822	WP_001234426.1	DUF945 domain-containing protein	-
H7R10_RS23495	34128..34730	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H7R10_RS23500	35051..35434	+	384	WP_001151524.1	relaxosome protein TraM	-
H7R10_RS23505	35621..36310	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / blaCTX-M-65 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	iucA / iucB / iucC / iucD / iutA	1..105973	105973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T33806 WP_001312861.1 NZ_AP021934:31526-31684 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T33806 NZ_AP021934:31526-31684 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT33806 NZ_AP021934:c31477-31414 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin