Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2330505..2331067 Replicon chromosome
Accession NZ_AP021933
Organism Escherichia coli strain WP2-W18-ESBL-08

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag H7R10_RS10830 Protein ID WP_000605676.1
Coordinates 2330789..2331067 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H7R10_RS10825 Protein ID WP_000781370.1
Coordinates 2330505..2330789 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R10_RS10810 2325864..2328911 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
H7R10_RS10815 2328924..2329808 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
H7R10_RS10820 2329801..2330454 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
H7R10_RS10825 2330505..2330789 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H7R10_RS10830 2330789..2331067 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R10_RS10835 2331253..2332263 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
H7R10_RS10840 2332397..2334094 - 1698 WP_000433464.1 malate dehydrogenase -
H7R10_RS10845 2334251..2334388 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H7R10_RS10850 2334490..2334705 - 216 WP_000495766.1 biofilm-dependent modulation protein -
H7R10_RS10855 2335050..2335481 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T33788 WP_000605676.1 NZ_AP021933:c2331067-2330789 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T33788 NZ_AP021933:c2331067-2330789 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT33788 WP_000781370.1 NZ_AP021933:c2330789-2330505 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT33788 NZ_AP021933:c2330789-2330505 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References