Detailed information of TA system TA033779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 122482..123156
• Replicon: plasmid pWP2-W18-ESBL-06_2
• Accession: NZ_AP021931
• Organism: Klebsiella pneumoniae strain WP2-W18-ESBL-06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: H7R50_RS26280
• Protein ID: WP_032720638.1
• Coordinates: 122833..123156 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R50_RS26275
• Protein ID: WP_032720637.1
• Coordinates: 122482..122784 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R50_RS26235	117675..118247	+	573	WP_044266753.1	hypothetical protein	-
H7R50_RS26240	118278..118772	+	495	WP_044266755.1	hypothetical protein	-
H7R50_RS26245	119075..119995	+	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
H7R50_RS26250	120031..120819	+	789	WP_032720700.1	TSUP family transporter	-
H7R50_RS26255	120821..121393	+	573	WP_032720635.1	GNAT family N-acetyltransferase	-
H7R50_RS26260	121498..121881	+	384	WP_032720636.1	hypothetical protein	-
H7R50_RS26265	121905..122084	+	180	WP_032720701.1	hypothetical protein	-
H7R50_RS26270	122272..122439	+	168	WP_153591440.1	hypothetical protein	-
H7R50_RS26275	122482..122784	-	303	WP_032720637.1	helix-turn-helix domain-containing protein	Antitoxin
H7R50_RS26280	122833..123156	-	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R50_RS26285	123334..123886	-	553	Protein_133	DUF4113 domain-containing protein	-
H7R50_RS26290	124213..124815	-	603	Protein_134	transposase	-
H7R50_RS26295	125316..125639	-	324	WP_019725650.1	hypothetical protein	-
H7R50_RS26300	125712..126707	-	996	WP_019725651.1	hypothetical protein	-
H7R50_RS26305	127004..127654	-	651	WP_015065500.1	SOS response-associated peptidase	-
H7R50_RS26310	127701..128099	-	399	Protein_138	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A)	-	1..130287	130287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T33779 WP_032720638.1 NZ_AP021931:c123156-122833 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T33779 NZ_AP021931:c123156-122833 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT33779 WP_032720637.1 NZ_AP021931:c122784-122482 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT33779 NZ_AP021931:c122784-122482 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5