Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3712207..3712804 Replicon chromosome
Accession NZ_AP021929
Organism Klebsiella pneumoniae strain WP2-W18-ESBL-06

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag H7R50_RS18190 Protein ID WP_004142563.1
Coordinates 3712487..3712804 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag H7R50_RS18185 Protein ID WP_004142561.1
Coordinates 3712207..3712494 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R50_RS18155 3708287..3708535 + 249 WP_020325243.1 DUF1158 domain-containing protein -
H7R50_RS18160 3708553..3708894 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
H7R50_RS18165 3708925..3710040 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
H7R50_RS18170 3710220..3710801 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
H7R50_RS18175 3710801..3711169 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
H7R50_RS18180 3711289..3711942 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
H7R50_RS18185 3712207..3712494 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
H7R50_RS18190 3712487..3712804 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R50_RS18195 3712989..3714032 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
H7R50_RS18200 3714702..3715568 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
H7R50_RS18205 3715677..3717104 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T33770 WP_004142563.1 NZ_AP021929:c3712804-3712487 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T33770 NZ_AP021929:c3712804-3712487 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT33770 WP_004142561.1 NZ_AP021929:c3712494-3712207 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT33770 NZ_AP021929:c3712494-3712207 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References