Detailed information of TA system TA033756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 78152..78804
• Replicon: plasmid pWP2-W18-ESBL-01_1
• Accession: NZ_AP021928
• Organism: Aeromonas caviae strain WP2-W18-ESBL-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R60_RS22930
• Protein ID: WP_182936403.1
• Coordinates: 78152..78502 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R60_RS22935
• Protein ID: WP_182936404.1
• Coordinates: 78502..78804 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R60_RS22895	73501..74403	-	903	WP_182936398.1	reverse transcriptase	-
H7R60_RS22900	74413..74799	+	387	WP_182936399.1	four helix bundle protein	-
H7R60_RS22905	75006..75374	-	369	WP_182936400.1	Txe/YoeB family addiction module toxin	-
H7R60_RS22910	75262..75507	-	246	WP_182936401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H7R60_RS22915	75591..75695	-	105	Protein_68	recombinase family protein	-
H7R60_RS22920	76100..77170	+	1071	Protein_69	IS21 family transposase	-
H7R60_RS22925	77321..77935	+	615	WP_182936402.1	recombinase family protein	-
H7R60_RS22930	78152..78502	+	351	WP_182936403.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R60_RS22935	78502..78804	+	303	WP_182936404.1	XRE family transcriptional regulator	Antitoxin
H7R60_RS22940	79045..80181	+	1137	WP_182935169.1	ISAs1 family transposase	-
H7R60_RS22945	80411..81223	+	813	WP_182936405.1	ParA family protein	-
H7R60_RS22950	81229..81513	+	285	WP_182936406.1	hypothetical protein	-
H7R60_RS22955	81913..83166	+	1254	WP_042650430.1	IS256-like element ISAeme25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135252	135252
	-	inside	IScluster/Tn	-	-	72129..96071	23942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-89)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12982.85 Da
• Isoelectric Point: 6.4664

>T33756 WP_182936403.1 NZ_AP021928:78152-78502 [Aeromonas caviae]
MWTVKTTDVFDTWFDAQDNATQEKVLAGLLALAQGGPSVGRPLVDTIKGSSFTNLKELRVQHQGEPLRAFFAFDPLRQAI
VLCAGNKGGNEKRFYKEMLPIAEAEYARHLAELEMR

Nucleotide

• Length: 351 bp

>T33756 NZ_AP021928:78152-78502 [Aeromonas caviae]
ATGTGGACAGTAAAAACGACCGATGTGTTTGATACTTGGTTCGACGCTCAAGACAATGCTACGCAGGAAAAGGTATTGGC
CGGGTTGCTGGCTTTGGCGCAAGGTGGCCCATCGGTAGGGCGCCCTTTGGTTGATACCATTAAGGGTTCCAGCTTTACGA
ACCTCAAAGAGCTGAGAGTTCAGCACCAGGGAGAACCGCTGCGGGCCTTCTTCGCTTTTGATCCGTTACGACAAGCAATT
GTGCTGTGTGCCGGCAATAAGGGCGGTAACGAGAAGAGGTTTTACAAAGAAATGTTGCCGATTGCTGAGGCTGAATACGC
CAGGCATTTAGCTGAATTGGAGATGCGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11060.81 Da
• Isoelectric Point: 5.1768

>AT33756 WP_182936404.1 NZ_AP021928:78502-78804 [Aeromonas caviae]
MGKTLTEMLASRSADSQQRIKDMADELMLEVRLQAVREELEVSQVELASALGITQPAIVAMEQRGSDVKLSTMKRYVEAM
GGKLRVDVELPTGRHIGFNV

Nucleotide

• Length: 303 bp

>AT33756 NZ_AP021928:78502-78804 [Aeromonas caviae]
ATGGGAAAAACACTGACTGAAATGCTAGCCTCCCGCTCGGCGGATAGCCAGCAACGTATCAAGGATATGGCTGACGAGCT
GATGCTGGAAGTACGCTTGCAAGCTGTGCGCGAAGAGCTAGAGGTCTCCCAGGTGGAATTAGCCTCAGCACTCGGTATCA
CACAACCAGCTATTGTGGCGATGGAGCAGCGCGGTAGCGATGTCAAACTATCCACAATGAAGCGCTACGTGGAGGCTATG
GGTGGCAAGCTGCGTGTGGATGTAGAGCTTCCTACAGGACGACATATCGGCTTCAACGTTTGA