Detailed information of TA system TA033734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4556985..4557587
• Replicon: chromosome
• Accession: NZ_AP021914
• Organism: Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7Q98_RS21810
• Protein ID: WP_000897305.1
• Coordinates: 4557276..4557587 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7Q98_RS21805
• Protein ID: WP_000356397.1
• Coordinates: 4556985..4557275 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7Q98_RS21780	4552911..4553813	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7Q98_RS21785	4553810..4554445	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7Q98_RS21790	4554442..4555371	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
H7Q98_RS21795	4555701..4555943	-	243	WP_001086388.1	hypothetical protein	-
H7Q98_RS21800	4556162..4556380	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7Q98_RS21805	4556985..4557275	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7Q98_RS21810	4557276..4557587	-	312	WP_000897305.1	hypothetical protein	Toxin
H7Q98_RS21815	4557816..4558724	+	909	WP_182912522.1	alpha/beta hydrolase	-
H7Q98_RS21820	4558788..4559729	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
H7Q98_RS21825	4559774..4560211	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7Q98_RS21830	4560208..4561080	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7Q98_RS21835	4561074..4561673	-	600	WP_124783521.1	glucose-1-phosphatase	-
H7Q98_RS21840	4561772..4562557	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T33734 WP_000897305.1 NZ_AP021914:c4557587-4557276 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T33734 NZ_AP021914:c4557587-4557276 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT33734 WP_000356397.1 NZ_AP021914:c4557275-4556985 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT33734 NZ_AP021914:c4557275-4556985 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7