Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3704289..3704830 Replicon chromosome
Accession NZ_AP021914
Organism Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag H7Q98_RS17815 Protein ID WP_000615982.1
Coordinates 3704552..3704830 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H7Q98_RS17810 Protein ID WP_000729704.1
Coordinates 3704289..3704549 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q98_RS17785 3700072..3700857 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
H7Q98_RS17790 3700829..3702541 + 1713 Protein_3482 flagellar biosynthesis protein FlhA -
H7Q98_RS17795 3702646..3702924 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H7Q98_RS17800 3702917..3703273 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H7Q98_RS17805 3703330..3704103 - 774 WP_000543899.1 C40 family peptidase -
H7Q98_RS17810 3704289..3704549 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H7Q98_RS17815 3704552..3704830 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H7Q98_RS17820 3704986..3705726 + 741 WP_001225679.1 murein L,D-transpeptidase -
H7Q98_RS17825 3705697..3706464 - 768 WP_000333380.1 class II glutamine amidotransferase -
H7Q98_RS17830 3706670..3707248 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T33732 WP_000615982.1 NZ_AP021914:3704552-3704830 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T33732 NZ_AP021914:3704552-3704830 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT33732 WP_000729704.1 NZ_AP021914:3704289-3704549 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT33732 NZ_AP021914:3704289-3704549 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References