Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1752701..1753203 Replicon chromosome
Accession NZ_AP021914
Organism Escherichia coli strain WP2-W18-CRE-03

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7Q98_RS08265 Protein ID WP_000767829.1
Coordinates 1752949..1753203 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag H7Q98_RS08260 Protein ID WP_001610372.1
Coordinates 1752701..1752952 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q98_RS08235 1747879..1748946 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7Q98_RS08240 1748946..1750016 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
H7Q98_RS08245 1750013..1751317 - 1305 WP_001367667.1 histidinol dehydrogenase -
H7Q98_RS08250 1751323..1752222 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
H7Q98_RS08255 1752368..1752418 - 51 WP_001364200.1 his operon leader peptide -
H7Q98_RS08260 1752701..1752952 + 252 WP_001610372.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7Q98_RS08265 1752949..1753203 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7Q98_RS08270 1753286..1754110 + 825 WP_000754737.1 SDR family oxidoreductase -
H7Q98_RS08275 1754156..1755085 + 930 WP_000803366.1 LysR family transcriptional regulator -
H7Q98_RS08280 1755300..1755362 + 63 WP_010723108.1 membrane protein YoeI -
H7Q98_RS08285 1755352..1756710 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
H7Q98_RS08290 1756890..1757948 + 1059 WP_000492330.1 transport protein YeeE -
H7Q98_RS08295 1757962..1758189 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33720 WP_000767829.1 NZ_AP021914:1752949-1753203 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33720 NZ_AP021914:1752949-1753203 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9289.55 Da        Isoelectric Point: 4.7954

>AT33720 WP_001610372.1 NZ_AP021914:1752701-1752952 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLIDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33720 NZ_AP021914:1752701-1752952 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATAGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure

References