Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3874351..3874948 Replicon chromosome
Accession NZ_AP021909
Organism Klebsiella pneumoniae strain WP2-S17-ESBL-02

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag H7R06_RS18740 Protein ID WP_004142563.1
Coordinates 3874631..3874948 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag H7R06_RS18735 Protein ID WP_004142561.1
Coordinates 3874351..3874638 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R06_RS18705 3870432..3870680 + 249 WP_002893037.1 DUF1158 domain-containing protein -
H7R06_RS18710 3870698..3871039 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
H7R06_RS18715 3871070..3872185 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
H7R06_RS18720 3872364..3872945 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
H7R06_RS18725 3872945..3873313 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
H7R06_RS18730 3873433..3874086 + 654 Protein_3677 oxygen-insensitive NAD(P)H nitroreductase -
H7R06_RS18735 3874351..3874638 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
H7R06_RS18740 3874631..3874948 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R06_RS18745 3875133..3876176 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
H7R06_RS18750 3876843..3877709 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
H7R06_RS18755 3877818..3879245 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T33709 WP_004142563.1 NZ_AP021909:c3874948-3874631 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T33709 NZ_AP021909:c3874948-3874631 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT33709 WP_004142561.1 NZ_AP021909:c3874638-3874351 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT33709 NZ_AP021909:c3874638-3874351 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References