Detailed information of TA system TA033687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5004214..5004868
• Replicon: chromosome
• Accession: NZ_AP021904
• Organism: Pseudomonas sp. Ost2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Y8CB69
• Locus tag: KI243_RS21820
• Protein ID: WP_042937104.1
• Coordinates: 5004214..5004564 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI243_RS21825
• Protein ID: WP_177118801.1
• Coordinates: 5004554..5004868 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI243_RS21805 (PHLH7_43060)	4999986..5001017	-	1032	WP_017129435.1	GlxA family transcriptional regulator	-
KI243_RS21810 (PHLH7_43070)	5001048..5002709	-	1662	WP_178990817.1	choline dehydrogenase	-
KI243_RS21815 (PHLH7_43080)	5002772..5003941	-	1170	WP_178990815.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
KI243_RS21820 (PHLH7_43090)	5004214..5004564	+	351	WP_042937104.1	hypothetical protein	Toxin
KI243_RS21825 (PHLH7_43100)	5004554..5004868	+	315	WP_177118801.1	transcriptional regulator	Antitoxin
KI243_RS21830 (PHLH7_43110)	5005017..5005958	+	942	WP_177118800.1	cupin	-
KI243_RS21835 (PHLH7_43120)	5005965..5006885	-	921	WP_177086687.1	LysR family transcriptional regulator	-
KI243_RS21840 (PHLH7_43130)	5006930..5008198	-	1269	WP_177086685.1	cyanide-forming glycine dehydrogenase subunit HcnC	-
KI243_RS21845 (PHLH7_43140)	5008202..5009599	-	1398	WP_212809904.1	cyanide-forming glycine dehydrogenase subunit HcnB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13792.74 Da
• Isoelectric Point: 9.0757

>T33687 WP_042937104.1 NZ_AP021904:5004214-5004564 [Pseudomonas sp. Ost2]
MNALFIELPPFERHRRDYLGDEAFRCLQLALLHNPEAGEVIQGTGGLRKVRCVDLQRHKGKRGGIRVIYYRWPQGSQFWL
FTLYDKDERDDLSPQQKHILKQLLNREIEARNHHET

Nucleotide

• Length: 351 bp

>T33687 NZ_AP021904:5004214-5004564 [Pseudomonas sp. Ost2]
ATGAATGCTTTGTTTATCGAACTGCCGCCCTTCGAACGCCATCGCAGAGACTACCTGGGCGACGAGGCATTCCGCTGTCT
CCAGCTCGCCTTGCTGCACAACCCAGAAGCAGGCGAGGTCATCCAGGGCACCGGCGGGCTGCGCAAGGTCCGTTGCGTTG
ACCTGCAACGACACAAAGGCAAACGAGGCGGCATCCGGGTGATCTACTACAGGTGGCCGCAAGGCTCCCAGTTCTGGCTA
TTCACCCTGTACGACAAGGACGAACGGGATGATTTGAGTCCACAGCAGAAGCACATCCTGAAACAACTGCTGAACAGAGA
GATAGAGGCGAGAAACCACCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11742.56 Da
• Isoelectric Point: 10.6081

>AT33687 WP_177118801.1 NZ_AP021904:5004554-5004868 [Pseudomonas sp. Ost2]
MKRDIFAELVEGFDALADQRQGKVTLRTHKVQLGKLQPISADELVAIRTQLKLSRAVFALYLRTNTRTLENWEQGRARPN
AQATTLIRLVEKYPETVGHLASLG

Nucleotide

• Length: 315 bp

>AT33687 NZ_AP021904:5004554-5004868 [Pseudomonas sp. Ost2]
ATGAAACGTGACATTTTTGCAGAACTGGTCGAGGGCTTCGATGCACTGGCCGACCAGCGCCAGGGGAAAGTCACCCTGCG
TACCCACAAGGTCCAACTGGGCAAGCTGCAACCGATCAGCGCCGATGAACTGGTGGCGATCCGCACCCAGTTGAAACTGT
CTCGGGCGGTGTTCGCCCTGTACCTGCGCACCAACACCCGGACCCTGGAGAACTGGGAGCAGGGCCGCGCCAGGCCCAAT
GCCCAGGCCACCACGCTGATCCGGCTGGTGGAGAAGTACCCCGAAACCGTCGGGCACCTGGCCTCCCTGGGCTGA

Structures

Toxin

• AlphaFold DB: A0A7Y8CB69