Detailed information of TA system TA033676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1087982..1088637
• Replicon: chromosome
• Accession: NZ_AP021902
• Organism: Pseudomonas sp. Cab53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0D0PKE3
• Locus tag: KI241_RS04640
• Protein ID: WP_042728258.1
• Coordinates: 1087982..1088326 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI241_RS04645
• Protein ID: WP_042728259.1
• Coordinates: 1088323..1088637 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI241_RS04615 (PHLH5_09150)	1082998..1083945	+	948	WP_042728255.1	ubiquinol oxidase subunit II	-
KI241_RS04620 (PHLH5_09160)	1083949..1085979	+	2031	WP_042728256.1	cytochrome o ubiquinol oxidase subunit I	-
KI241_RS04625 (PHLH5_09170)	1085983..1086609	+	627	WP_042728257.1	cytochrome o ubiquinol oxidase subunit III	-
KI241_RS04630 (PHLH5_09180)	1086610..1086945	+	336	WP_025211927.1	cytochrome o ubiquinol oxidase subunit IV	-
KI241_RS04635 (PHLH5_09190)	1086957..1087844	+	888	WP_025211928.1	heme o synthase	-
KI241_RS04640 (PHLH5_09200)	1087982..1088326	+	345	WP_042728258.1	hypothetical protein	Toxin
KI241_RS04645 (PHLH5_09210)	1088323..1088637	+	315	WP_042728259.1	helix-turn-helix domain-containing protein	Antitoxin
KI241_RS04650 (PHLH5_09220)	1088848..1089552	-	705	WP_042728260.1	hypothetical protein	-
KI241_RS04655 (PHLH5_09230)	1089691..1090434	-	744	WP_042728261.1	DUF72 domain-containing protein	-
KI241_RS04660 (PHLH5_09240)	1090501..1091514	-	1014	WP_212803859.1	sensor domain-containing diguanylate cyclase	-
KI241_RS04665 (PHLH5_09250)	1091629..1092546	-	918	WP_212803427.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13598.59 Da
• Isoelectric Point: 9.2897

>T33676 WP_042728258.1 NZ_AP021902:1087982-1088326 [Pseudomonas sp. Cab53]
MHTLFFETTAFTATVGHYLTDDEYRLLQSYLLQHPTAGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDLQFWM
FAIYDKDELENLTREQEKSLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T33676 NZ_AP021902:1087982-1088326 [Pseudomonas sp. Cab53]
ATGCATACCTTATTTTTTGAAACGACTGCGTTCACCGCCACGGTGGGACATTACCTGACTGACGATGAATATCGTTTGCT
CCAGTCCTATCTGCTGCAGCACCCAACGGCCGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAGCGTCGTGGCAAGGGGAAGCGAGGTGGTTTGCGAGTCATTTACTATTGGCTCATGAATGATCTTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCCGCGAGCAAGAGAAGTCGCTCAAGCGCGCCATTGAGGCTGA
GTTGAAAGTGCGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11813.76 Da
• Isoelectric Point: 9.9445

>AT33676 WP_042728259.1 NZ_AP021902:1088323-1088637 [Pseudomonas sp. Cab53]
MKKRDLFAEMMQGIEEMAAHREGKLTLRQVSIEDKPAPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSRP
NAQAALLIRLVEKFPDMVERLAAV

Nucleotide

• Length: 315 bp

>AT33676 NZ_AP021902:1088323-1088637 [Pseudomonas sp. Cab53]
ATGAAAAAACGTGATCTGTTTGCCGAGATGATGCAGGGCATCGAGGAAATGGCCGCTCACCGGGAGGGCAAGCTGACCCT
TCGCCAGGTATCGATCGAAGATAAACCAGCGCCCGAGGTATCGGCCCAGGAAATTGTGGCGTTGCGTGACAAGCTCCACA
TGTCCCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCTGGCACGCTCAGGAACTGGGAGCAGGAAAAGTCCAGGCCC
AATGCCCAGGCGGCTCTGTTGATCAGGTTGGTCGAGAAGTTTCCAGACATGGTCGAGCGGCTCGCAGCTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0D0PKE3