Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 834506..835098 Replicon chromosome
Accession NZ_AP021902
Organism Pseudomonas sp. Cab53

Toxin (Protein)


Gene name higB Uniprot ID A0A0D0PFE1
Locus tag KI241_RS03520 Protein ID WP_042728179.1
Coordinates 834820..835098 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A0D0PR32
Locus tag KI241_RS03515 Protein ID WP_042728178.1
Coordinates 834506..834811 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI241_RS03490 (PHLH5_06930) 830740..831012 + 273 WP_042728199.1 hypothetical protein -
KI241_RS03495 831148..831267 - 120 Protein_688 RNA 2'-phosphotransferase -
KI241_RS03500 (PHLH5_06940) 831840..832182 + 343 Protein_689 DUF6124 family protein -
KI241_RS03505 (PHLH5_06950) 832239..832784 - 546 WP_212803379.1 RNA 2'-phosphotransferase -
KI241_RS03510 (PHLH5_06960) 833005..834486 + 1482 WP_212803380.1 leucine-rich repeat domain-containing protein -
KI241_RS03515 (PHLH5_06970) 834506..834811 - 306 WP_042728178.1 HigA family addiction module antitoxin Antitoxin
KI241_RS03520 (PHLH5_06980) 834820..835098 - 279 WP_042728179.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KI241_RS03525 (PHLH5_06990) 835272..837644 - 2373 WP_212803381.1 TonB-dependent siderophore receptor -
KI241_RS03530 (PHLH5_07000) 837766..838746 - 981 WP_212803382.1 DUF4880 domain-containing protein -
KI241_RS03535 (PHLH5_07010) 838733..839248 - 516 WP_042728200.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 823210..845478 22268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-97)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10422.92 Da        Isoelectric Point: 8.5301

>T33675 WP_042728179.1 NZ_AP021902:c835098-834820 [Pseudomonas sp. Cab53]
MIRSFKCADTQALFETGKARRWAAILSVAERKLGMLNAAVELNDLRSPPGNRLEPLQGDRQGQHSIRINGQYRICFVWGI
NGPEQVEIVDYH

Download         Length: 279 bp

>T33675 NZ_AP021902:c835098-834820 [Pseudomonas sp. Cab53]
ATGATCCGAAGCTTCAAATGCGCCGACACCCAAGCCCTTTTTGAAACCGGCAAGGCCCGGCGCTGGGCCGCTATCCTTTC
AGTTGCGGAACGAAAGCTGGGGATGCTCAACGCAGCGGTCGAACTCAATGACCTCCGCTCCCCACCGGGAAACAGGCTGG
AGCCGCTGCAGGGCGACAGACAGGGTCAGCACAGCATTCGGATCAATGGTCAGTACCGAATCTGTTTTGTCTGGGGCATC
AATGGCCCCGAACAGGTAGAAATCGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 7.2768

>AT33675 WP_042728178.1 NZ_AP021902:c834811-834506 [Pseudomonas sp. Cab53]
MFKNGMRPVHPGEILKEEYLEPLGLSAAALARALHVSAPTINDIVLKRRSISADVALRLAAALDTSAQFWLNLQIAYDLR
TAEIAKGEQIYREVEKVPHCA

Download         Length: 306 bp

>AT33675 NZ_AP021902:c834811-834506 [Pseudomonas sp. Cab53]
ATGTTTAAGAACGGCATGAGGCCTGTACATCCCGGGGAAATTCTCAAAGAAGAATACCTGGAACCACTTGGCCTTTCGGC
AGCCGCGCTTGCAAGGGCTTTGCACGTTTCGGCCCCCACGATAAATGACATTGTGTTGAAGCGTCGCAGCATCAGCGCAG
ATGTTGCCTTGCGCTTGGCAGCCGCGTTGGATACGTCAGCGCAATTTTGGCTGAACCTGCAGATTGCCTATGATTTACGT
ACAGCCGAGATTGCTAAAGGTGAGCAGATCTACCGGGAAGTGGAGAAGGTTCCTCACTGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D0PFE1

Antitoxin

Source ID Structure
AlphaFold DB A0A0D0PR32

References