Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 4544005..4544609 Replicon chromosome
Accession NZ_AP021901
Organism Pseudomonas sp. St316

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KI237_RS20230 Protein ID WP_212796766.1
Coordinates 4544005..4544319 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KI237_RS20235 Protein ID WP_212796767.1
Coordinates 4544322..4544609 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI237_RS20210 (PHLH4_40330) 4539469..4541040 - 1572 WP_212796762.1 GGDEF domain-containing protein -
KI237_RS20215 (PHLH4_40340) 4541251..4541910 - 660 WP_212796763.1 ribonuclease T2 -
KI237_RS20220 (PHLH4_40350) 4542088..4543446 + 1359 WP_212796764.1 chromate efflux transporter -
KI237_RS20225 (PHLH4_40360) 4543443..4543874 - 432 WP_212796765.1 arsenic resistance N-acetyltransferase ArsN2 -
KI237_RS20230 (PHLH4_40370) 4544005..4544319 + 315 WP_212796766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KI237_RS20235 (PHLH4_40380) 4544322..4544609 + 288 WP_212796767.1 helix-turn-helix domain-containing protein Antitoxin
KI237_RS20240 (PHLH4_40390) 4544754..4545242 + 489 WP_212796768.1 hypothetical protein -
KI237_RS20245 (PHLH4_40400) 4545288..4545524 - 237 WP_212796769.1 hypothetical protein -
KI237_RS20250 (PHLH4_40410) 4545669..4546886 - 1218 WP_212796770.1 MFS transporter -
KI237_RS20255 (PHLH4_40420) 4547219..4548127 + 909 WP_212796771.1 AraC family transcriptional regulator -
KI237_RS20260 (PHLH4_40430) 4548399..4549247 - 849 WP_212796772.1 nickel/cobalt efflux protein RcnA -
KI237_RS20265 (PHLH4_40440) 4549255..4549542 - 288 WP_212796773.1 metal-sensing transcriptional repressor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(42-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-87)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11947.06 Da        Isoelectric Point: 9.9011

>T33673 WP_212796766.1 NZ_AP021901:4544005-4544319 [Pseudomonas sp. St316]
MSFIETPIFTRRVKELMDDDDFAVLQKILVCNPCAGDVMEATGGIRKIRVAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKVLKMIVEQWR

Download         Length: 315 bp

>T33673 NZ_AP021901:4544005-4544319 [Pseudomonas sp. St316]
ATGAGTTTTATTGAAACTCCAATTTTTACCCGTCGGGTCAAAGAGCTGATGGATGACGATGACTTCGCTGTCCTGCAGAA
AATACTGGTCTGCAATCCTTGCGCTGGTGATGTGATGGAAGCCACCGGGGGGATCCGAAAGATTCGTGTCGCAGCCAAAG
GCCATGGCAAACGGGGTGGGGCCAGGGTGATCTACTACCACTTCGTCCATGCTTCGCGGATCGCGTTATTGATGATATAT
CCGAAAAATGAACAGCAAGACTTGACTGTCGATCAACGCAAAGTATTGAAAATGATTGTCGAGCAATGGAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10810.50 Da        Isoelectric Point: 10.0845

>AT33673 WP_212796767.1 NZ_AP021901:4544322-4544609 [Pseudomonas sp. St316]
MSKFFDELMERVQQMDEIVRGKRQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPEHVISALAV

Download         Length: 288 bp

>AT33673 NZ_AP021901:4544322-4544609 [Pseudomonas sp. St316]
ATGAGCAAATTTTTCGATGAGCTGATGGAAAGAGTACAACAGATGGATGAAATCGTCCGAGGCAAACGCCAGCCCTCACG
GGAGTTCATCGTCGATGCGTTGCAAGTGAAAGAAATACGCAAGGCTACCGGCCTGACCCAGGCCAAATTCGCAGCACTGA
TCGACGTCCAATTGGGCACGCTTCGCAATTGGGAGCAAGGACGACGCGAGCCAACCGGCCCCGCTAAAGCGTTGCTGCGA
GCGATTCATAATGATCCCGAACACGTGATCAGCGCCCTGGCAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References