Detailed information of TA system TA033671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5644650..5645305
• Replicon: chromosome
• Accession: NZ_AP021900
• Organism: Pseudomonas sp. St386

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: KI235_RS24930
• Protein ID: WP_013694185.1
• Coordinates: 5644961..5645305 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI235_RS24925
• Protein ID: WP_003205391.1
• Coordinates: 5644650..5644964 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI235_RS24905 (PHLH3_49710)	5640696..5641613	+	918	WP_003205387.1	LysR family transcriptional regulator	-
KI235_RS24910 (PHLH3_49720)	5641763..5642776	+	1014	WP_003205388.1	sensor domain-containing diguanylate cyclase	-
KI235_RS24915 (PHLH3_49730)	5642868..5643611	+	744	WP_003205389.1	DUF72 domain-containing protein	-
KI235_RS24920 (PHLH3_49740)	5643733..5644437	+	705	WP_003205390.1	hypothetical protein	-
KI235_RS24925 (PHLH3_49750)	5644650..5644964	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
KI235_RS24930 (PHLH3_49760)	5644961..5645305	-	345	WP_013694185.1	hypothetical protein	Toxin
KI235_RS24935 (PHLH3_49770)	5645445..5646332	-	888	WP_003205393.1	heme o synthase	-
KI235_RS24940 (PHLH3_49780)	5646344..5646679	-	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
KI235_RS24945 (PHLH3_49790)	5646680..5647306	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
KI235_RS24950 (PHLH3_49800)	5647310..5649340	-	2031	WP_003205396.1	cytochrome o ubiquinol oxidase subunit I	-
KI235_RS24955 (PHLH3_49810)	5649344..5650291	-	948	WP_003205397.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T33671 WP_013694185.1 NZ_AP021900:c5645305-5644961 [Pseudomonas sp. St386]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T33671 NZ_AP021900:c5645305-5644961 [Pseudomonas sp. St386]
ATGCGTACCCTATTTTTTGAAACGACTACATTCACCGACACGGTTGGCCATTACCTGACTGATGATGAGTATCGCCTGCT
TCAGTCCTATATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAACGTCGTGGTAAAGGGAAGCGAGGCGGGTTGCGCGTTATTTACTATTGGCTCATGAACGATCGTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAACGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT33671 WP_003205391.1 NZ_AP021900:c5644964-5644650 [Pseudomonas sp. St386]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT33671 NZ_AP021900:c5644964-5644650 [Pseudomonas sp. St386]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCCCACCGGGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCTGCCCAAGAAATCGTGGCGTTGCGCGACAAGCTCCACA
TGTCTCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACGCTCAGGAACTGGGAGCAGGAGAAGTCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAGCTGGTCGAGAAGTTTCCAGATATGGTCGAACGGCTTGGCGCTGTTTGA

Structures

Toxin

• AlphaFold DB: G8QAX9