Detailed information of TA system TA033668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3872035..3872576
• Replicon: chromosome
• Accession: NZ_AP021896
• Organism: Escherichia coli strain 2018-02-2CC

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: HRR25_RS18410
• Protein ID: WP_000615980.1
• Coordinates: 3872035..3872313 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HRR25_RS18415
• Protein ID: WP_000729707.1
• Coordinates: 3872316..3872576 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR25_RS18395	3869617..3870195	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
HRR25_RS18400	3870401..3871168	+	768	WP_000333380.1	class II glutamine amidotransferase	-
HRR25_RS18405	3871139..3871879	-	741	WP_001225679.1	murein L,D-transpeptidase	-
HRR25_RS18410	3872035..3872313	-	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HRR25_RS18415	3872316..3872576	-	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HRR25_RS18420	3872762..3873535	+	774	WP_000543885.1	C40 family peptidase	-
HRR25_RS18425	3873711..3874208	+	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
HRR25_RS18430	3874441..3876153	-	1713	Protein_3585	flagellar biosynthesis protein FlhA	-
HRR25_RS18435	3876125..3876910	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T33668 WP_000615980.1 NZ_AP021896:c3872313-3872035 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T33668 NZ_AP021896:c3872313-3872035 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT33668 WP_000729707.1 NZ_AP021896:c3872576-3872316 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT33668 NZ_AP021896:c3872576-3872316 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGACCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A090NH48