Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1142737..1143239 Replicon chromosome
Accession NZ_AP021896
Organism Escherichia coli strain 2018-02-2CC

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag HRR25_RS05630 Protein ID WP_000767826.1
Coordinates 1142737..1142991 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRR25_RS05635 Protein ID WP_001259255.1
Coordinates 1142988..1143239 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR25_RS05600 1137752..1137979 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HRR25_RS05605 1137993..1139051 - 1059 WP_033808140.1 transport protein YeeE -
HRR25_RS05610 1139230..1140588 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HRR25_RS05615 1140578..1140640 - 63 WP_010723108.1 membrane protein YoeI -
HRR25_RS05620 1140855..1141784 - 930 WP_000803363.1 LysR family transcriptional regulator -
HRR25_RS05625 1141830..1142654 - 825 WP_000754737.1 SDR family oxidoreductase -
HRR25_RS05630 1142737..1142991 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRR25_RS05635 1142988..1143239 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRR25_RS05640 1143522..1143572 + 51 WP_001364200.1 his operon leader peptide -
HRR25_RS05645 1143718..1144617 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HRR25_RS05650 1144623..1145927 + 1305 WP_001350692.1 histidinol dehydrogenase -
HRR25_RS05655 1145924..1146994 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
HRR25_RS05660 1146994..1148061 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T33662 WP_000767826.1 NZ_AP021896:c1142991-1142737 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33662 NZ_AP021896:c1142991-1142737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33662 WP_001259255.1 NZ_AP021896:c1143239-1142988 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33662 NZ_AP021896:c1143239-1142988 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References