Detailed information of TA system TA033649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3603446..3604140
• Replicon: chromosome
• Accession: NZ_AP021895
• Organism: Escherichia coli strain 2018-11-3CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HRR26_RS17370
• Protein ID: WP_001263493.1
• Coordinates: 3603446..3603844 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HRR26_RS17375
• Protein ID: WP_000554757.1
• Coordinates: 3603847..3604140 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR26_RS17340	3598446..3599690	-	1245	WP_000189541.1	esterase FrsA	-
-	3599106..3599186	-	81	NuclAT_9	-	-
-	3599106..3599186	-	81	NuclAT_9	-	-
-	3599106..3599186	-	81	NuclAT_9	-	-
-	3599106..3599186	-	81	NuclAT_9	-	-
HRR26_RS17345	3599782..3600240	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HRR26_RS17350	3600501..3601958	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HRR26_RS17355	3602015..3602536	-	522	Protein_3392	peptide chain release factor H	-
HRR26_RS17360	3602535..3602738	-	204	Protein_3393	RNA ligase RtcB family protein	-
HRR26_RS17365	3602984..3603436	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HRR26_RS17370	3603446..3603844	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HRR26_RS17375	3603847..3604140	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HRR26_RS17380	3604192..3605244	-	1053	WP_000691315.1	DNA polymerase IV	-
HRR26_RS17385	3605306..3606850	+	1545	WP_115735755.1	recombinase family protein	-
HRR26_RS17390	3606888..3607850	+	963	WP_152952776.1	hypothetical protein	-
HRR26_RS17395	3607854..3608426	-	573	WP_000086540.1	recombinase family protein	-
HRR26_RS17400	3608795..3609088	-	294	WP_000896590.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3592963..3627332	34369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T33649 WP_001263493.1 NZ_AP021895:c3603844-3603446 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33649 NZ_AP021895:c3603844-3603446 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT33649 WP_000554757.1 NZ_AP021895:c3604140-3603847 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33649 NZ_AP021895:c3604140-3603847 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6