33637

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1756788..1757290 Replicon chromosome
Accession NZ_AP021895
Organism Escherichia coli strain 2018-11-3CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HRR26_RS08295 Protein ID WP_000767829.1
Coordinates 1757036..1757290 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRR26_RS08290 Protein ID WP_001259255.1
Coordinates 1756788..1757039 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR26_RS08265 1751967..1753034 - 1068 WP_001610379.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HRR26_RS08270 1753034..1754104 - 1071 WP_001614841.1 histidinol-phosphate transaminase -
HRR26_RS08275 1754101..1755405 - 1305 WP_001515511.1 histidinol dehydrogenase -
HRR26_RS08280 1755411..1756310 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
HRR26_RS08285 1756456..1756506 - 51 WP_001364200.1 his operon leader peptide -
HRR26_RS08290 1756788..1757039 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRR26_RS08295 1757036..1757290 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRR26_RS08300 1757373..1758197 + 825 WP_000754749.1 SDR family oxidoreductase -
HRR26_RS08305 1758243..1759172 + 930 WP_000803351.1 LysR family transcriptional regulator -
HRR26_RS08310 1759387..1759449 + 63 WP_010723108.1 membrane protein YoeI -
HRR26_RS08315 1759439..1760797 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HRR26_RS08320 1760976..1762034 + 1059 WP_000492305.1 transport protein YeeE -
HRR26_RS08325 1762048..1762275 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33637 WP_000767829.1 NZ_AP021895:1757036-1757290 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33637 NZ_AP021895:1757036-1757290 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33637 WP_001259255.1 NZ_AP021895:1756788-1757039 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33637 NZ_AP021895:1756788-1757039 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References