Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1756788..1757290 Replicon chromosome
Accession NZ_AP021895
Organism Escherichia coli strain 2018-11-3CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HRR26_RS08295 Protein ID WP_000767829.1
Coordinates 1757036..1757290 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRR26_RS08290 Protein ID WP_001259255.1
Coordinates 1756788..1757039 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR26_RS08265 1751967..1753034 - 1068 WP_001610379.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HRR26_RS08270 1753034..1754104 - 1071 WP_001614841.1 histidinol-phosphate transaminase -
HRR26_RS08275 1754101..1755405 - 1305 WP_001515511.1 histidinol dehydrogenase -
HRR26_RS08280 1755411..1756310 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
HRR26_RS08285 1756456..1756506 - 51 WP_001364200.1 his operon leader peptide -
HRR26_RS08290 1756788..1757039 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRR26_RS08295 1757036..1757290 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRR26_RS08300 1757373..1758197 + 825 WP_000754749.1 SDR family oxidoreductase -
HRR26_RS08305 1758243..1759172 + 930 WP_000803351.1 LysR family transcriptional regulator -
HRR26_RS08310 1759387..1759449 + 63 WP_010723108.1 membrane protein YoeI -
HRR26_RS08315 1759439..1760797 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HRR26_RS08320 1760976..1762034 + 1059 WP_000492305.1 transport protein YeeE -
HRR26_RS08325 1762048..1762275 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33637 WP_000767829.1 NZ_AP021895:1757036-1757290 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33637 NZ_AP021895:1757036-1757290 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33637 WP_001259255.1 NZ_AP021895:1756788-1757039 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33637 NZ_AP021895:1756788-1757039 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References