Detailed information of TA system TA033628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3674092..3674786
• Replicon: chromosome
• Accession: NZ_AP021894
• Organism: Escherichia coli strain 2018-10-1CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HRR23_RS17975
• Protein ID: WP_001263493.1
• Coordinates: 3674092..3674490 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HRR23_RS17980
• Protein ID: WP_000554757.1
• Coordinates: 3674493..3674786 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR23_RS17945	3669092..3670336	-	1245	WP_000189541.1	esterase FrsA	-
-	3669752..3669832	-	81	NuclAT_8	-	-
-	3669752..3669832	-	81	NuclAT_8	-	-
-	3669752..3669832	-	81	NuclAT_8	-	-
-	3669752..3669832	-	81	NuclAT_8	-	-
HRR23_RS17950	3670428..3670886	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HRR23_RS17955	3671147..3672604	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HRR23_RS17960	3672661..3673182	-	522	Protein_3510	peptide chain release factor H	-
HRR23_RS17965	3673181..3673384	-	204	Protein_3511	RNA ligase RtcB family protein	-
HRR23_RS17970	3673630..3674082	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HRR23_RS17975	3674092..3674490	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HRR23_RS17980	3674493..3674786	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HRR23_RS17985	3674838..3675893	-	1056	WP_172410268.1	DNA polymerase IV	-
HRR23_RS17990	3675964..3676749	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HRR23_RS17995	3676721..3678433	+	1713	Protein_3517	flagellar biosynthesis protein FlhA	-
HRR23_RS18000	3678657..3679154	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T33628 WP_001263493.1 NZ_AP021894:c3674490-3674092 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33628 NZ_AP021894:c3674490-3674092 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT33628 WP_000554757.1 NZ_AP021894:c3674786-3674493 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33628 NZ_AP021894:c3674786-3674493 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6