Detailed information of TA system TA033607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3738350..3739044
• Replicon: chromosome
• Accession: NZ_AP021893
• Organism: Escherichia coli strain 2018-06-4CC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HRR20_RS18065
• Protein ID: WP_001263493.1
• Coordinates: 3738350..3738748 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HRR20_RS18070
• Protein ID: WP_000554757.1
• Coordinates: 3738751..3739044 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR20_RS18035	3733350..3734594	-	1245	WP_000189541.1	esterase FrsA	-
-	3734010..3734090	-	81	NuclAT_10	-	-
-	3734010..3734090	-	81	NuclAT_10	-	-
-	3734010..3734090	-	81	NuclAT_10	-	-
-	3734010..3734090	-	81	NuclAT_10	-	-
HRR20_RS18040	3734686..3735144	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HRR20_RS18045	3735405..3736862	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HRR20_RS18050	3736919..3737440	-	522	Protein_3536	peptide chain release factor H	-
HRR20_RS18055	3737439..3737642	-	204	Protein_3537	RNA ligase RtcB family protein	-
HRR20_RS18060	3737888..3738340	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HRR20_RS18065	3738350..3738748	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HRR20_RS18070	3738751..3739044	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HRR20_RS18075	3739096..3740151	-	1056	WP_001226164.1	DNA polymerase IV	-
HRR20_RS18080	3740222..3741007	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HRR20_RS18085	3740979..3742691	+	1713	Protein_3543	flagellar biosynthesis protein FlhA	-
HRR20_RS18090	3742915..3743412	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3688363..3763619	75256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T33607 WP_001263493.1 NZ_AP021893:c3738748-3738350 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T33607 NZ_AP021893:c3738748-3738350 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT33607 WP_000554757.1 NZ_AP021893:c3739044-3738751 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT33607 NZ_AP021893:c3739044-3738751 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6