Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1778688..1779190 Replicon chromosome
Accession NZ_AP021893
Organism Escherichia coli strain 2018-06-4CC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HRR20_RS08315 Protein ID WP_000767829.1
Coordinates 1778936..1779190 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRR20_RS08310 Protein ID WP_001259255.1
Coordinates 1778688..1778939 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR20_RS08285 1773866..1774933 - 1068 WP_021547024.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HRR20_RS08290 1774933..1776003 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
HRR20_RS08295 1776000..1777304 - 1305 WP_001567685.1 histidinol dehydrogenase -
HRR20_RS08300 1777310..1778209 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HRR20_RS08305 1778355..1778405 - 51 WP_001364200.1 his operon leader peptide -
HRR20_RS08310 1778688..1778939 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRR20_RS08315 1778936..1779190 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRR20_RS08320 1779273..1780097 + 825 WP_000754737.1 SDR family oxidoreductase -
HRR20_RS08325 1780143..1781072 + 930 WP_000803351.1 LysR family transcriptional regulator -
HRR20_RS08330 1781287..1781349 + 63 WP_010723108.1 membrane protein YoeI -
HRR20_RS08335 1781339..1782697 + 1359 WP_024198437.1 putrescine/proton symporter PlaP -
HRR20_RS08340 1782876..1783934 + 1059 WP_000492305.1 transport protein YeeE -
HRR20_RS08345 1783948..1784175 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T33594 WP_000767829.1 NZ_AP021893:1778936-1779190 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T33594 NZ_AP021893:1778936-1779190 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT33594 WP_001259255.1 NZ_AP021893:1778688-1778939 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT33594 NZ_AP021893:1778688-1778939 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References